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[en]
 Decision making in large fruit fly sterile insect technique (SIT) programs, a process that involves massive releases of sterile flies, depends largely on a continuous feedback on the area-wide achievement of sterile to wild ratios. Uncertainties faced in these programs have often focused on the adequacy of the marking system and the availability of an accurate technique to discriminate between marked sterile and unmarked wild flies captured in monitoring traps. In the study described here, data were collected over several years by the Mexico/United States Mediterranean fruit fly eradication/containment program (Programa Moscamed) in southern Mexico and Guatemala, we compared the traditional system of detecting marked sterile flies with a UV lamp with a system based on fluorescent microscopy. Our results indicate that fluorescent microscopy is more accurate, faster, and less expensive than use of traditional UV light system. Numbers of sterile flies without discernible dye per million flies captured decreased by approximately 75%, representing a 4-fold reduction in dissection costs. Use of fluorescent microscopy also reduced uncertainty due to unidentifiable flies and therefore reduced the potential of declared outbreaks that require disruptive and costly emergency action. Success in large scale SIT eradication/containment programs is ultimately determined by the detection of pockets of few remaining wild flies among thousands of recaptured mass released sterile flies. Thus use of fluorescent microscopy will allow for a more rapid and accurate diagnosis and results in a more effective reaction to real fly outbreaks in eradication or fly free zones


SubjectAPPLIED LIFE SCIENCES (S60)
Source/ReportJournal of economic entomology; v. 89(4); ISSN 0022-0493; 1996; p. 946-949; ARN: US19970062856; Country of input: International Atomic Energy Agency (IAEA)
Record TypeJournal article
Country/Org.United States
DECANIMALS; ARTHROPODS; CONTROL; DIPTERA; ELECTROMAGNETIC RADIATION; EMISSION; FLIES; FOOD; FRUIT FLIES; INSECTS; INVERTEBRATES; LUMINESCENCE; PHOTON EMISSION; RADIATIONS
DEIACCURACY; CERATITIS CAPITATA; DECISION MAKING; FLUORESCENCE; FRUITS; MICROSCOPY; PEST CONTROL; STERILE INSECT RELEASE; STERILE MALE TECHNIQUE; ULTRAVIOLET RADIATION
LanguageEnglish
Ref. Number45060861
Publ. Year1996
INIS Volume45
INIS Issue23
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