Effects of cadmium on soil nitrification in the rhizosphere of Robinia pseudoacacia L. seedlings under elevated atmospheric CO2 scenarios

Highlights: • Elevated CO₂ increased root biomass of seedlings exposed to Cd at days 90 and 135. • Elevated CO₂ decreased Cd content and increased pH in rhizospheric soils. • Elevated CO₂ stimulated AMO and HAO activities under low Cd levels. • Elevated CO₂ stimulated NXR activity in Cd-contaminated rhizosphere soils. • Elevated CO₂ altered nitrifier abundance and community structure exposed to Cd. The individual impacts of elevated CO₂ and heavy metals on soil nitrification have been widely reported. However, studies on the combined effects of elevated CO₂ and heavy metals on soil nitrification are still limited. Here, a 135-day growth chamber experiment was conducted to investigate the impacts of elevated CO₂ and cadmium (Cd) levels on soil nitrification in the rhizosphere of Robinia pseudoacacia L. seedlings. Elevated CO₂ combined with Cd pollution generally stimulated ammonia monooxygenase (AMO), hydroxylamine oxidase (HAO), and nitrite oxidoreductase (NXR) activities. Compared to the control, the abundance of ammonia-oxidizing bacteria (AOB) at day 135 and ammonia-oxidizing archaea (AOA) increased significantly (p < 0.05) and the abundance of AOB at days 45 and 90 and that of the nitrite-oxidizing bacteria (NOB) decreased under elevated CO₂ + Cd. Elevated CO₂ mostly led to a significant (p < 0.05) decrease in soil nitrification intensity in the rhizosphere of R. pseudoacacia exposed to Cd. The effects of Cd, CO₂, and their interaction on HAO and NXR activities were significant (p < 0.01). Soil pH, the C/N ratio, water-soluble organic carbon, water-soluble organic nitrogen (WSON), and total carbon were the dominant factors (p < 0.05) affecting nitrifying enzyme activities and nitrification intensity in rhizosphere soils. Elevated CO₂ clearly affected AOA, AOB, and NOB community structures and dominant genera by shaping C/N ratio, pH, and Cd and WSON contents in rhizosphere soils under Cd exposure. Overall, the responses of pH, C/N ratio, WSON, and Cd to elevated CO₂ led to changes in rhizosphere soil nitrification under the combination of elevated CO₂ and Cd pollution.