Published 2000 | Version v1
Report Open

The neutral comet assay

Creators

  • 1. Institute of Nuclear Chemistry and Technology, Warsaw (Poland)

Description

The single-cell gel electrophoresis (or comet) assay is a rapid and sensitive fluorescence microscopic method that is used for the detection of DNA damage at the individual cell level. Comet assay under neutral conditions allows the detection of DNA double-strand breaks, considered to the biologically relevant radiation-induced lesion. In this report we describe modifications of the neutral comet method and its use for studying DNA damage and its repair in irradiated human lymphocytes. (author)

Availability note (English)

Available from INIS in electronic form

Files

32032373.pdf

Files (467.2 kB)

Name Size Download all
md5:20e565f896ba2d2c4dd416d148e2ffa2
467.2 kB Preview Download

Additional details

Additional titles

Original title (Polish)
Test kometowy w pH obojetnym
Augmented title (English)
29 refs, 7 figs, 1 tab

Publishing Information

Imprint Pagination
18 p.
Report number
INCT--2/B/2000

INIS

Country of Publication
Poland
Country of Input or Organization
Poland
INIS RN
32032373
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; DNA DAMAGES; DNA REPAIR; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; LYMPHOCYTES; MAN; STRAND BREAKS
Descriptors DEC
ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DNA DAMAGES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LEUKOCYTES; MAMMALS; MATERIALS; PRIMATES; RADIATION EFFECTS; RADIATIONS; REPAIR; SOMATIC CELLS; VERTEBRATES

Optional Information

Notes
of irradiated lymphocytes