Phosphorus elevation erodes ectomycorrhizal community diversity and induces divergence of saprophytic community composition between vegetation types

Highlights: • Phosphorus (P) is an indispensable macronutrient for all biota on earth. • P elevation erodes ectomycorrhizal community diversity. • P level induces divergence of microbial community structure between the tree types. • Microbes and P level interactively influence the tree health. Phosphorus (P) is a critical macronutrient that is essential for many life-sustaining processes. Despite decades of work on plant performance under P deficiency and the importance of microbes in ecosystem processes, little is known about how bacterial and fungal flora respond to P gradients and determine the vegetation health. In current study, we examined soil edaphic conditions and microbial communities in 39 untouched natural forests representing phosphorous deficient (Pp) and phosphorus rich (Pr) soils (due to naturally occurring phosphate rocks) in Yunnan Province, China. We also considered the effect of plant functional types by including the dominant tree species. Bacterial and fungal diversity was greater across the Pp sites compared with Pr sites. The relative abundance of Actinobacteria and Gemmatimonadetes was higher across Pp sites, while Chlamydiae and Verrucomicrobia showed the opposite pattern, with greater relative abundance across the Pr sites. Bacterial taxa that were observed in low P soils were more likely having oligotrophic life history strategies. Interestingly, ectomycorrhizal (ECM) fungal diversity was promoted in the Pp sites, indicating that the decreasing soil P concentration and the increasing host P demand foster stimulated the ECM species for hyphal soil exploration. Moreover, the high P level caused saprophytic fungi (SAP) to diverge, causing its enrichment only under Q. variabilis compared to low P soil, where there is no difference in relative abundance of SAP between the two tree species. This likely resulted in an enhanced decomposition process by SAP and elevation of soil properties (Carbon and Nitrogen) under Q. variabilis across the Pr sites. Taken together, our findings highlight the highly diverse microbiome in low P soils. The higher soil P caused shifts of fungal functional guilds, which likely influence tree growth and health (ECM), along with divergence of ecosystem services between tree functional types.