Published May 2006 | Version v1
Journal article Metadata-only

Quantitative evaluation of magnetic immunoassay with remanence measurement

Creators

  • 1. Research Institute of Superconductor Science and Systems, Kyushu University, Fukuoka 812-8581 (Japan)
  • 2. Department of Medical Science, Kyushu University, Fukuoka 812-8582 (Japan)
  • 3. Advanced Research Laboratory, Hitachi, Ltd, Kokubunji-shi, Tokyo 185-8601 (Japan)
  • 4. Central Research Laboratory, Hitachi, Ltd, Kokubunji-shi, Tokyo 185-8601 (Japan)

Description

Magnetic immunoassays utilizing magnetic markers and a high -Tc SQUID have been performed. The marker was designed so as to generate remanence, and its remanence field was measured with the SQUID. The SQUID system was developed so as to measure 12 samples in one measurement sequence. We first conducted a detection of antigen called human IgE using IgE standard solution, and showed the detection of IgE down to 2 attomol. The binding process between IgE and the marker could be semi-quantitatively explained with the Langmuir-type adsorption model. We also measured IgE in human serums, and demonstrated the usefulness of the present method for practical diagnosis

Availability note (English)

Available online at http://stacks.iop.org/0953-2048/19/S257/sust6_5_S19.pdf or at the Web site for the journal Superconductor Science and Technology (ISSN 1361-6668) http://www.iop.org/

Additional details

Identifiers

URL
http://stacks.iop.org/0953-2048/19/S257/sust6_5_S19.pdf;
DOI
10.1088/0953-2048/19/5/S19;
PII
S0953-2048(06)06415-3;

Publishing Information

Journal Title
Superconductor Science and Technology
Journal Volume
19
Journal Issue
5
Journal Page Range
p. S257-S260
ISSN
0953-2048
CODEN
SUSTEF

Conference

Title
10. international superconductive electronics conference
Acronym
ISEC 2005
Dates
5-9 Sep 2005
Place
Noordwijkerhout (Netherlands)

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
37085126
Subject category
S36: MATERIALS SCIENCE;
Resource subtype / Literary indicator
Conference
Descriptors DEI
ADSORPTION; ANTIGENS; DETECTION; DIAGNOSIS; EVALUATION; HIGH-TC SUPERCONDUCTORS; IMMUNOASSAY; SQUID DEVICES
Descriptors DEC
BIOASSAY; ELECTRONIC EQUIPMENT; EQUIPMENT; FLUXMETERS; MEASURING INSTRUMENTS; MICROWAVE EQUIPMENT; SORPTION; SUPERCONDUCTING DEVICES; SUPERCONDUCTORS; TYPE-II SUPERCONDUCTORS