Published November 24, 1982
| Version v1
Journal article
Radioimmunoassay of ricin A- and B-chains applied to samples of ricin A-chain prepared by chromatofocusing and by DEAE Bio-Gel A chromatography
Creators
- 1. Kansas Univ., Lawrence (USA). Dept. of Biochemistry
Description
A radioimmunoassay for ricin and ricin A- and B-chains was developed. Amounts as low as 100 pg of A-chain and 500 pg of B-chain could easily be quantitated. We showed, however, that the free chains were more reactive in the radioimmunoassay than the equivalent quantity of the individual chains when combined in intact ricin. The usefulness of the assay was demonstrated by determining the concentration of contaminating A- or B-chains in preparations of the separate polypeptides purified by DEAE Bio-Gel A chromatography and by chromatofocusing. (orig.)
Additional details
Publishing Information
- Journal Title
- Biochim. Biophys. Acta - Gen. Subj.
- Journal Volume
- 719
- Journal Issue
- 2
- Series
- CODEN: BBGSB.;Biochim. Biophys. Acta - Gen. Subj.
- Journal Page Range
- 341-348
- ISSN
- 0304-4165
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 14746023
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CHROMATOGRAPHY; COMPLEXES; IODINE 125; LABELLING; RADIOIMMUNOASSAY; TOXINS
- Descriptors DEC
- ANTIGENS; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPES; SEPARATION PROCESSES; TRACER TECHNIQUES