Published August 1993 | Version v1
Journal article

Technetium-99m labelled LDL as a tracer for quantitative LDL scintigraphy. Pt. 1

  • 1. Vienna Univ. (Austria). Clinic of Nuclear Medicine
  • 2. Vienna Univ. (Austria). Dept. of Medical Chemistry
  • 3. Austrian Research Center, Seibersdorf (Austria)

Description

The goal of the present study was to optimize technetium-99m labelling of low-density lipoprotein (LDL) and to investigate the in vitro and in vivo properties of the tracer to determine whether its application for quantitative scintigraphy of hepatic LDL receptor activity is feasible. Despite a high radiochemical purity immediately after labelling, modifications of 99mTc labelling of LDL did not overcome the poor stability of the tracer. Subsequent dialysis in phosphate buffer increased the stability in vitro and in vivo. It was concluded that dialysis substantially increases stability, which is essential for quantification purposes, and that the dialysed tracer retains its biological integrity as it is recognized by the LDL receptor in vitro

Additional details

Additional titles

Subtitle (English)
Tracer purification, in vitro and in vivo long-term stability, in vitro validation and biodistribution

Publishing Information

Journal Title
European Journal of Nuclear Medicine
Journal Volume
20
Journal Issue
8
Journal Page Range
p. 667-673.
ISSN
0340-6997
CODEN
EJNMD9