Published November 13, 1980 | Version v1
Journal article

Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells

  • 1. National Inst. for Medical Research, London (UK)

Description

In combination with high performance liquid chromatography methods using reverse-phase C18 columns, convenient and sensitive radioimmune (RI) and radiobinding (RB) assays have been used to detect core and 2-5A in crude extracts from interferon-treated cells. The novel 2-5A synthetase products NAD2'p5'A2'p5'A and A5'p45'A2'p5'A2'p5'A which can also be detected using the RB assay, were not found in significant amounts. (U.K.)

Additional details

Publishing Information

Journal Title
Nature (London)
Journal Volume
288
Journal Issue
5787
Series
Nature (London).
Journal Page Range
189-192
ISSN
0028-0836

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
12585152
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ANIMAL CELLS; BIOLOGICAL LOCALIZATION; INTERFERON; LIGASES; LIQUID COLUMN CHROMATOGRAPHY; RADIOIMMUNOLOGY
Descriptors DEC
CHROMATOGRAPHY; ENZYMES; IMMUNOLOGY; ORGANIC COMPOUNDS; SEPARATION PROCESSES