Published 1983
| Version v1
Journal article
Barbiturate N-demethylase activity in isolated rat hepatocytes
Description
The isolated hepatocyte preparation has become an increasingly popular system for studying xenobiotic metabolism, and there is a need for methods of determining metabolic activity. Described here is a simple and sensitive radiometric assay for barbiturate N-demethylase activity in the isolated hepatocyte suspension. The demethylation of 1,3-dimethylphenobarbital is determined by measuring the formation of its only significant metabolite, N-methylphenobarbital. The reaction is a typical P-450-mediated dealkylation
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Volume
- 129
- Series
- Anal. Biochem.
- Journal Page Range
- 235-244
- ISSN
- 0003-2697
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 15013391
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CARBON 14 COMPOUNDS; DEALKYLATION; ENZYMES; LIVER CELLS; METABOLISM; PHENOBARBITAL; RADIOMETRIC ANALYSIS
- Descriptors DEC
- ANIMAL CELLS; AZINES; BARBITURATES; CARBON COMPOUNDS; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; DRUGS; HETEROCYCLIC COMPOUNDS; HYPNOTICS AND SEDATIVES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PYRIMIDINES; QUANTITATIVE CHEMICAL ANALYSIS; SOMATIC CELLS