Published 2004 | Version v1
Miscellaneous Metadata-only

Inhibition of Rad51 via RNA interference leads to MMC sensitisation but not radiation sensitisation in CHO cells

Creators

  • 1. Institut fuer Biophysik und Strahlenbiologie, Universitaetsklinikum Eppendorf Hamburg (Germany)

Description

This study was dedicated to examining the effect of inhibition of Rad51 protein through RNA interference on the sensitivity to mitomycin C and ionising radiation in a cell line with intact NHEJ (CHO-K1) as compared with a cell line with defective NHEJ (xrs5). In adult cells inhibition of Rad51 protein through RNA interference has no effect on radiation sensitivity. It follows that Rad51 only plays a minor role in the repair of DNA double strand breaks in cells with intact NHEJ

Abstract (German)

In dieser Arbeit wurde untersucht, welchen Effekt die Hemmung des Rad51-Proteins via RNA-Interferenz auf eine Zelllinie mit intaktem NHEJ (CHO-K1) im Vergleich zu einer Linie mit defektem NHEJ (xrs5) auf die Empfindlichkeit gegenueber Mitomycin C und ionisierende Bestrahlung hat. Die Inhibition des RAD51-Proteins durch RNA-Interferenz hat in adulten Zellen keinen Effekt auf die Strahlenempfindlichkeit. Folglich spielt Rad51 nur eine untergeordnete Rolle in der Reparatur von DNA-Doppelstrangbruechen in Zellen mit funktionellem NHEJ. (orig.)
Part of:
Experimental radiotherapy and clinical radiobiology. Vol. 13

Additional details

Additional titles

Original title (German)
Die Inhibition von RAD51 via RNA-Interferenz bewirkt eine MMC- aber keine Strahlensensibilisierung in CHO-Zellen

Publishing Information

Imprint Title
Experimental radiotherapy and clinical radiobiology. Vol. 13
Imprint Pagination
140 p.
Journal Page Range
p. 8-12
ISSN
1432-864X

Conference

Title
13. symposium on experimental radiotherapy and clinical radiobiology
Original Conference Title
13. Symposium Experimentelle Strahlentherapie und Klinische Strahlenbiologie
Dates
4-6 Mar 2004
Place
Dresden (Germany)

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
36046374
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Conference, Non-conventional Literature
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CELL CULTURES; CHO CELLS; EXTERNAL IRRADIATION; GENE REGULATION; IN VITRO; INHIBITION; MITOMYCIN; PROTEINS; RADIOSENSITIVITY; RNA; SURVIVAL CURVES; X RADIATION
Descriptors DEC
ANIMAL CELLS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIMITOTIC DRUGS; ANTINEOPLASTIC DRUGS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; IRRADIATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; REPAIR; SOMATIC CELLS

Optional Information

Notes
Imprint:Experimentelle Strahlentherapie und Klinische Strahlenbiologie. Bd. 13. Proceedings