Studies of cell kinetics in vital and necrotic tumor regions: Measurement of loss rate using 125iododeoxyuridine
Description
The method of determining cell losses in situ from the decrease in radioactivity after a single i.p. injection of the thymidine-analogue, 125iododeoxyuridine was tested for its applicability in different development stages of the solid mammary adenocarcinoma EO 771 on male C57 N1/6J mice. Invasive studies on activity distributions in vital and necrotic tumour regions were carried out after injection of Light Green SF. The following results were obtained: 1) External measurement of activity loss register the removal of labelled cells and decay products rather than cell death. The total activity of the tumour at any time following injection corresponds to the sum of radioactivity in the proliferating, non-growing and necrotic compartments. 2) In solid tumours, there are regions with differing transport capacity. 3) The method using 125J-UdR does not allow determination of loss rates as a function of a given volume but rather yields an averaged value. 4) Activity losses of vital tumour cells largely correspond to the cell loss. (orig./MG)
Availability note (English)
MF available from INIS under the Report Number.Abstract (German)
Die Methode, Zellverluste in-situ aus dem Abfall der Radioaktivitaet nach einer einzelnen intraperitonealen Injektion des Thymidinanalogons 125Joddesoxyuridin zu ermitteln, wurde waehrend verschiedener Phasen der Tumorentwicklung des soliden Mammaadenocarcinoms EO 771 auf maennlichen C57 Bl/6J Maeusen auf ihre Anwendbarkeit getestet. Invasive Untersuchungen ueber die Aktivitaetsverteilung in vitalen und nektrotischen Tumorbereichen wurden nach Injektion des Farbstoffes Lichtgruen SF durchgefuehrt. Aus der vorliegenden Untersuchung lassen sich folgende Schluesse ziehen: 1. Die externe Messung des Aktivitaetsverlustes erfasst eher den Abtransport markierter Zellen und ihrer Abbauprodukte als den Zelltod. Die Gesamtaktivitaet des Tumors entspricht zu jedem Zeitpunkt nach Injektion der Summe der Aktivitaeten in profilierenden, ruhenden und nektrotischen Kompartments. 2. In soliden Tumoren bestehen Bereiche mit unterschiedlicher Abtransportkapazitaet. 3. Die 125J-UdR Methode ermittelt nicht eine bestimmte Verlustrate als Funktion eines bestimmten Volumes, sondern einen Mittelwert. 4. Aktivitaetsverluste vitaler Tumorzellen entsprechen weitgehend dem Zellverlust. (orig./MG)
Files
Restricted
The record is publicly accessible, but files are restricted to users with access.
Additional details
Additional titles
- Original title (German)
- Zellkinetische Untersuchungen in vitalen und nekrotischen Tumorbereichen: Anwendbarkeit der Verlustratenmessung mit
Publishing Information
- Imprint Pagination
- 145 p.
- Report number
- INIS-mf--8276
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 14792229
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Thesis
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; CARCINOMAS; CELL KILLING; CELL PROLIFERATION; EXPERIMENTAL NEOPLASMS; IODINE 125; IODODEOXYURIDINE; LABELLED COMPOUNDS; MEASURING METHODS; MICE; TRACER TECHNIQUES; TUMOR CELLS
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; ANTIMETABOLITES; ANTIMITOTIC DRUGS; AZINES; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; IODOURACILS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; NEOPLASMS; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIOISOTOPES; REACTION KINETICS; RIBOSIDES; RODENTS; URACILS; VERTEBRATES