Construction of dose response curves up to 6 Gy for Micronucleus and Dicentric Chromosome Aberration Assay with 6 MV X-ray Beam

Highlights: • Large scale bio-dosimetry is enforced for the advent LINAC usage emergency concern. • Calibration curves were plotted by CBMN and DCA assays for 6 MV X-ray beam with the dose rate of 300 cGy/min. • Compared manually constructed curves with CABAS and Dose estimation programs. • Linear (α) and Quadratic ($\beta$) co-efficient variation was found between up to 4 Gy and 6 Gy constructed graph. • Accuracy of the dose estimation was verified for constructed calibration curves. In-vitro dose response curves were built with 6 MV X-ray for a dose range of 0.1–6 Gy, at a dose rate of 300 cGy/min, using Linear Accelerator (LINAC, SIMENS PRIMUS) for Cytokinesis-Block Micronucleus (CBMN) and Dicentric Chromosome Aberration assay (DCA). The dose response curves constructed up to 6 Gy and also up to 4 Gy using the manual method were compared to those curves obtained with the software program such as CABAS (V.2) and Dose Estimate software (V.5.2). The alpha and beta coefficients for MN and CA obtained for the curves constructed up to 6 Gy showed variation compared to those obtained in dose response curve constructed up to 4 Gy. However, the doses estimated based on these coefficients did not show much variation. The results are discussed in this paper.