Indirect Determination of Penicillamine using Fe(II)-Phenanthroline Spectrophotometry

Fe³⁺ can react with penicillamine to form Fe²⁺, the amount of Fe²⁺ is directly proportional to the addition of penicillamine in system, and then Fe²⁺ reacted with the chromogenic reagent of phenanthroline to form a stable complex which its maximum absorption wavelength is 511nm. The content of penicillamine is determinated by measuring the absorbance of the stable orange red complex. The method for the indirect determination of penicillamine using Fe(II)-phenanthroline spectrophotometry has been established. The method is used to the determination of penicillamine in tablets, and the results are satisfactory. (paper)