Radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 human lung adenocarcinoma cells
- 1. Department of Radiation Oncology, The Second Affiliated Hospital, Cancer Institute, Zhejiang Univ. School of Medicine, Hangzhou (China)
Description
Objective: To investigate the radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 cells in vitro. Methods: A549 human lung adenocarcinoma cell line was used in this study. Cell growth kinetics was determined using MTT assay. Cell survival was analyzed by clonogenic assay. The change of cell cycle was measured by flow cytometry. Results: LM-1685 inhibited the growth of A549 cells, showing a dose-dependent and timeldependent manner. LM-1685 (50 μmol/L), either with or without IL-1β, showed the radiosensitizing effects on A549 cells, and the sensitizing enhancement ratio (SER) was 1.12 and 1.06, respectively. LM-1685 (50 μmol/L) abrogated radiation-induced G2/M arrest of the tested A549 cells. Conclusions: Cyclooxygenase-2 selective inhibitor can enhance the radiosensitivity of A549 cell line. Abrogation of radiation-induced G2/M arrest could be part of the mechanism. (authors)
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 28
- Journal Issue
- 4
- Journal Page Range
- p. 323-326
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 41095421
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ADENOMAS; CARCINOMAS; CELL CYCLE; GROWTH; HUMAN POPULATIONS; IN VITRO; KINETICS; LUNGS; RADIOSENSITIVITY; TUMOR CELLS; X RADIATION
- Descriptors DEC
- ANIMAL CELLS; BODY; CARCINOMAS; DISEASES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; NEOPLASMS; ORGANS; POPULATIONS; RADIATIONS; RESPIRATORY SYSTEM; SENSITIVITY
Optional Information
- Notes
- 3 figs., 1 tab., 13 refs.