Protein and polymer immobilized La0.7Sr0.3MnO3 nanoparticles for possible biomedical applications

La0.7Sr0.3MnO3 (LSMO) is a mixed-valent room temperature ferromagnet with properties that are attractive for their applicability in biomedicine. We report, for the first time, immobilization of commonly used biocompatible molecules on LSMO nanoparticles, namely bovine serum albumin and dextran. The former was conjugated to LSMO using 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide (CDI) as a coupling agent while the latter was used without any coupler. These bioconjugated nanoparticles exhibit several properties that suggest their applicability in the field of biomedicine, namely (a) no changes in the Curie temperature at ∼360 K after conjugation with biomolecules, (b) rapid attainment of the desired temperature (48 deg. C) at low concentration (e.g. fluidized dextran-coated system at 80 μg ml-1) upon exposure to 20 MHz radio-frequency, (c) extremely low cytotoxicity in skin carcinoma, human fibrosarcoma and neuroblastoma cell lines and (d) high stability of the LSMO system with negligible leaching of ionic manganese into the delivery medium, indicating their safety in possible human applications