Published March 1962
| Version v1
Book
A new biological method for preparing certain sulphurated substances labelled with S35
- 1. Centre d'Etudes Nucleaires de Saclay (France)
Description
Previous investigations have shown that the yolk-sac of embryonic birds' eggs can be used to produce the following reactions: (a) reduction of sulphate to sulphite; (b) fixation of the sulphite on the carbon chain produced by the desulfhydration of l-cysteine, with formation of l-cysteic acid; (c) decarboxylation of the l-cysteic acid into taurine. The enzymatic system which causes reaction (b) has been purified. It also acts as a catalyst in the sulphur-exchange between the cysteine and the mineral sulphide. The authors have utilized these data in preparing sulphurated substances labelled with S35: taurine S35, l-cysteine S35 and l-cysteic acid S35. For each of the three, they discuss the chemical reactions involved, the methods of preparation, the experimental conditions of extraction and purity-control, together with the yields and specific activities obtained. (author)
Abstract (French)
Des travaux anterieurs ont montre l'aptitude du sac vitellin d'oeufs embryonnes d'oiseaux a realiser les reactions suivantes: a) reduction du sulfate en sulfite, b) fixation du sulfite sur la chaine carbonee issue de la desulfhydration de la L-cysteine avec formation de l'acide L-cysteique, c) decarboxylation de l'acide L-cysteique en taurine. Le systeme enzymatique responsable de la reaction b a ete purifie ; il catalyse aussi l'echange du soufre de la cysteine avec celui du sulfure mineral. Les auteurs ont utilise ces donnees pour la preparation de substances soufrees marquees au 35S: taurine 35S, L-cystine 35S et acide L-cysteique 35S. Pour chacun de ces trois corps, ils decrivent les .reactions chimiques mises en jeu, les modes operatoires de fabrication, les conditions experimentales d'extraction et de controle de la purete, ainsi que les resultats obtenus tant pour les rendements que pour les activites specifiques obtenues. (author)Abstract (Spanish)
Trabajos anteriores han demostrado que la membrana vitelina de los huevos fecundados de pajaro es capaz de realizar las reacciones siguientes: a) reduccion de sulfatos a sulfites, b) fijacion de sulfito en la cadena de atomos de carbono resultante de la desulfhidratacion de la L-cisteina con formacion de acido L-cisteico, c) descarboxilacion del acido L-cisteico para dar taurina. Los autores han purificado el sistema enzimatico responsable de la reaccion b). Este sistema cataliza tambien el intercambio de azufre entre la cisterna y el sulfuro inorganico. Los autores se basan en estos datos para preparar sustancias azufradas marcadas con 35S: taurina-35S, L-cistina-35S y acido L-cisteico-35S. Describen las reacciones quimicas que intervienen en cada uno de los tres casos, asi como los metodos empleados en la sintesis, las condiciones experimentales de extraccion y de control de la pureza, y los resultados obtenidos en cuanto a rendimiento y actividades especificas alcanzados. (author)Abstract (Russian)
Predydushchie raboty pokazali sposobnost' vitellinovogo meshka ptich'ikh ehmbrional'nykh yaits osushchestvlyat' sleduyushchie reaktsii : a) vosstanovlenie sul'fata v sul'fit, b) fiksatsiyu sul'fita na uglerodnoj tsepi, proisshedshej ot sul'fgidratsii levovrashchayushchego tsisteina s obrazovaniem levovrashchayushchej tsisteinovoj kisloty, c) dekarboksilirovanie levovrashchayushchej tsisteinovoj kisloty v taurin. Ehnzimnaya sistema, otvetstvennaya za reaktsiyu ', byla ochishchena; ona takzhe kataliziruet obmen sery tsisteina s seroj sernistogo minerala. Avtory ispol'zovali ehti dannye dlya izgotovleniya serosoderzhashchikh veshchestv, mechennykh seroj-35: taurina S35, levovrashchayushchego tsistina i levovrashchayushchej tsisteinovoj kisloty S35. Dlya kazhdogo ehtogo veshchestva avtory opisyvayut dejstvuyushchie khimicheskie reaktsii, promyshlennye metody izgotovleniya, ehksperimental'nye usloviya ehkstraktsii i kontrolya chistoty, a takzhe rezul'taty, poluchennye kak dlya vykhodov, tak i dlya poluchennykh udel'nykh aktivnostej. (author)Additional details
Additional titles
- Original title (French)
- Nouvelle methode de preparation par voie biologique de quelques substances soufrees marquees au soufre-35; Novyj metod izgotovleniya biologicheskim putem nekotorykh serosoderzhashchikh veshchestv, mechennykh seroj-35; Nuevas biosintesis de algunos compuestos de azufre mareados con azufre-35
Publishing Information
- Publisher
- Globus, Druck- und Verlagsanstalt
- Imprint Place
- Vienna (International Atomic Energy Agency (IAEA))
- Imprint Title
- Radioisotopes in the Physical Sciences and Industry. Proceedings of the Conference on the Use of Radioisotopes in the Physical Sciences and Industry. V. III
- Imprint Pagination
- 652 p.
- Series
- Proceedings Series
- Journal Page Range
- p. 139-143
- ISSN
- 0074-1884
Conference
- Title
- Conference on the Use of Radioisotopes in the Physical Sciences and Industry
- Dates
- 6-17 Sep 1960
- Place
- Copenhagen (Denmark)
INIS
- Country of Publication
- International Atomic Energy Agency (IAEA)
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 43113011
- Subject category
- S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; S07: ISOTOPES AND RADIATION SOURCES;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- CATALYSTS; CONTROL; CYSTEINE; DECARBOXYLATION; EXTRACTION; IMPURITIES; MINERALS; REDUCTION; SULFATES; SULFIDES; SULFUR; TAURINE
- Descriptors DEC
- AMINES; AMINO ACIDS; CARBOXYLIC ACIDS; CHALCOGENIDES; CHEMICAL REACTIONS; ELEMENTS; NONMETALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXYGEN COMPOUNDS; SEPARATION PROCESSES; SULFONIC ACIDS; SULFUR COMPOUNDS; THIOLS
Optional Information
- Notes
- 6 refs
- Secondary number(s)
- STI/PUB--20(Vol.3)