Plasmid DNA lesions induced by α particle exposure observed with the atomic force microscopy
Creators
- 1. Academy of Military Medical Sciences, Beijing (China)
- 2. Academy of Atomic Energy Sciences, Beijing (China). Inst. of Nuclear Physics
Description
Objective: To examine the lesions of plasmid DNA induced by α particle exposure. Methods: The α-particle irradiated plasmid pGEM-Tl DNA was deposited on mica and was observed with Nanoscope III a atomic force microscopy. The irradiated DNA was concurrently analyzed by electrophoresis. Results: The three types of plasmid DNA molecules, i.e, open circle (OC), super coiled (SC) and linear were seen clearly under atomic force microscopy. The proportion of OC plasmid DNA was obviously increased at as less as 2 Gy of α particle exposure as compared with 10 Gy of γ-ray exposure. Conclusions: Clear image of plasmid DNA molecules was obtained using atomic force microscopy. The lesions of plasmid DNA induced by α particles were more serious than those by γ-ray with the same dose of exposure in vitro
Additional details
Publishing Information
- Journal Title
- Bulletin of the Academy of Military Medical Sciences
- Journal Volume
- 27
- Journal Issue
- 5
- Journal Page Range
- p. 334-335, 338
- ISSN
- 1000-5501
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 35074453
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ALPHA PARTICLES; ATOMIC FORCE MICROSCOPY; DNA; DOSE-RESPONSE RELATIONSHIPS; ELECTROPHORESIS; MOLECULAR STRUCTURE; PLASMIDS; RADIATION DOSES; RADIATION INJURIES
- Descriptors DEC
- BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; CELL CONSTITUENTS; CHARGED PARTICLES; DISEASES; DOSES; INJURIES; IONIZING RADIATIONS; MICROSCOPY; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS