Influence of AC electric field on the charge generation in albumin solution in a flow-based AFM fishing system

In the present paper, the influence of AC electric field on charge accumulation in the measuring cell of a flow-based system for atomic force microscope (AFM) based fishing is considered. As a flow-based system, a device for AFM-based fishing of low-abundant proteins has been employed. The charge is generated in a femtomolar albumin solution during its flowing through an injector of this system. We have demonstrated that application of sinusoidal electric field (100 V, 50 Hz frequency) stimulates charge accumulation in the measuring cell upon the input of protein solution at 38°C. Taking this effect into account is of importance for the development of novel highly sensitive flow-based proteomic and diagnostic systems employing AFM-based fishing, for modeling the influence of electric field on hemodynamics, and also for the development of specified theoretical models describing physicochemical properties of water and aqueous solutions. (paper)