Published October 12, 1990 | Version v1
Journal article Metadata-only

Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

Creators

  • 1. Univ. of California, San Francisco (USA)

Description

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes

Additional details

Publishing Information

Journal Title
Science (Washington, D.C.)
Journal Volume
250
Journal Issue
4978
Series
Science (Washington, D.C.).
Journal Page Range
245-250
ISSN
0036-8075
CODEN
SCIEA

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
22059807
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL MARKERS; CHROMOSOMES; DNA; ELECTROPHORESIS; GENES; GENETIC MAPPING; GENETIC RADIATION EFFECTS; MAN; SOMATIC MUTATIONS; X RADIATION
Descriptors DEC
ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; IONIZING RADIATIONS; MAMMALS; MUTATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; RADIATION EFFECTS; RADIATIONS; VERTEBRATES