Development of measuring method for platelet monoaminergic system for information transfer using radio-isotope and its clinical application

Aiming to develop a measuring method for various receptors such as σ, NMDA(N-methyl-D-aspartic acid) on human platelet using radioisotope (RI), quantitative determination method using RT-PCR for serotonin reuptake protein into human platelets was developed in the previous year. And quantitative determination for σ1 receptor was succeeded by amplifying the base sequence which recognizes the receptor using RT-PCR method. There kinds of primer, P1 P2 and P3 were constructed based on the base sequence obtained from Gen Bank and P2 was selected as the primer which allows most efficient expression. Neither of the three primers could detect the specific band for RNA extracted from rat platelet. There remains a possibility that since the base sequence for σ1 receptor is less homologous between the rat and human, its detection was unable by the primers used. (M.N.)