Antiproliferative and apoptosis-inducing effect of common Tunisian date seed (var. Korkobbi and Arechti) phytochemical-rich methanolic extract

In this study, the potential of date seed extracts to induce growth inhibition and apoptosis in HepG2 and HeLa cells was investigated. Analysis of the phytochemical compound content of the two Tunisian minor date seed extracts named Arechti and Korkobbi was determined. Moreover, their antioxidant properties are assessed through different assays including DPPH, ABTS, FRAP, TBARS, and phosphomolybdenum methods. Whereas, the cytotoxic effect was evaluated and apoptosis induction was confirmed by western blot technique (caspase-9, caspase-3, and PARP-1). The results proved the richness in phytochemical compounds of these by-products which explains the high in vitro antioxidant activity and the antiproliferative effects of both seed extracts. Additionally, the decrease in total PARP-1, procaspase-3 levels, and the increase of cleaved caspase-9 revealed the apoptotic effect of date seed extracts. These results collectively illustrate the potential of date seed extracts to induce growth inhibition and apoptosis in HepG2 and HeLa cells thanks to its phytochemical richness.