Investigation of phyotographic distribution of anti-P-gp antibody PHMA02 in nude mice

Objective: To investigate the photographic distribution of anti-P-gp monoclonal antibody PHMA02 in nude mice transplanted with K562/A02 cell tumor. Methods: PHMA02 were prepared by hybridoma technique, sodium dodecyl sulfate-polyacrylamide gel electrophorsis and Western-blot were used to analyzed its purity, fluorescence-activated cell sorting (FACS) was also used to determine its binding with K562 and K562/A02 cells. Nude mice were injected i.v with 125I labeled PHMA02, and were scanned on different time by SPECT. Results: The purity and binding affinity of PHMA02 were both satisfactory. On the second day from injection, radioactivity appeared in all tumor regions of the P-gp+(K562/A02) group nude mice, and intensified in the following days. Concludsion: The photographic difference of PHMA02 distribution between P-gp+(K562/A02) and P-gp-(K562) group could specifically distinguish the P-gp+ tumor tissue. (authors)