Published 1985
| Version v1
Book
Radio-immuno assays and ELISA for human leukocyte IFN-α
- 1. Weizman Inst. of Science, Rehovoth (Israel). Dept. of Virology
Description
An enzyme-linked-immunoabsorbent assay (ELISA) was developed for human leukocyte interferon (IFN-α) using monoclonal anti IFN-α as first, and rabbit polyclonal anti-IFN-α as second antibody. The bound antibody is then quantitated by alkaline phosphatase conjugated to goat anti-rabbit-IgG, and is proportional to the amount of IFN-α. Alternatively, [125I] protein A can be used to perform an immunoradiometric assay. The time required for these assays is about 7 hours. For more rapid detection a competitive radioimmunoassay was developed using rabbit polyclonal anti IFN-α antibodies and pure [125I] IFN-α. (Auth.)
Additional details
Publishing Information
- Publisher
- Elsevier.
- Imprint Place
- Amsterdam (Netherlands)
- ISBN
- 0-444-80515-x
- Imprint Title
- Immunoenzymatic techniques
- Imprint Pagination
- 420 p.
- Journal Volume
- 18
- Series
- Developments in Immunology.
- Journal Page Range
- p. 193-196.
Conference
- Title
- 2. International symposium on immunoenzymatic techniques.
- Dates
- 16-18 Mar 1983.
- Place
- Cannes (France).
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 17025123
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- ENZYME IMMUNOASSAY; INTERFERON; IODINE 125; LEUKOCYTES; PROTEINS; RADIOIMMUNOASSAY
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; TRACER TECHNIQUES
Optional Information
- Notes
- 5 refs.; 2 figs.; 1 table. Imprint:782 refs.; 166 figs.; 35 tabs.