Effect of Cytosine Arabinoside, 3-Aminobenzamide and Hydroxyurea on the frequencies of radiation-induced micronuclei and aneuploidy in human lymphocytes
- 1. Seoul National Univ., Seoul (Korea, Republic of)
- 2. Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of)
Description
This study was carried out to examine the effect of the DNA repair inhibitors, Cytosine Arabinoside(Ara C), 3-Aminobenzamide(3AB) and Hydroxyurea(HU) on the frequencies of radiation-induced MicroNuclei(MNi) and aneuploidy. Irradiated lymphocytes(1-3Gy) were treated with DNA repair inhibitors, Ara C, 3AB and HU for 3 hours and CBMN assay - FISH technique with DNA probe for chromosome 1 and 4 was performed. The frequencies of x-ray induced MNi and aneuploidy of chromosome 1 and 4 were increased in a dose-dependent manner. Ara C, 3AB and HU enhanced the frequencies of radiation-induced MNi and the frequencies of radiation-induced aneuploidy of chromosome 1 and 4 were enhanced by HU and Ara C while no effect was observed by 3AB. The frequency of radiation-induced aneuploidy of chromosome 1 was higher than that of chromosome 4. These results suggest that there are different mechanisms involved in the formation of MNi and aneuploidy by radiation
Additional details
Publishing Information
- Journal Title
- Journal of the Korean Association for Radiation Protection
- Journal Volume
- 30
- Journal Issue
- 4
- Series
- 42 refs, 4 figs, 4 tabs
- Journal Page Range
- p. 209-219
- ISSN
- 0253-4231
INIS
- Country of Publication
- Korea, Republic of
- Country of Input or Organization
- Korea, Republic of
- INIS RN
- 39096538
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANEUPLOIDY; ARABINOSE; CHROMOSOMES; CYTOSINE; HYDROXYUREA; LYMPHOCYTES
- Descriptors DEC
- ALDEHYDES; AMIDES; AMINES; ANIMAL CELLS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; LEUKOCYTES; MATERIALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PENTOSES; PLOIDY; PYRIMIDINES; SACCHARIDES; SOMATIC CELLS