Published February 1983
| Version v1
Journal article
Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex
- 1. National Inst. of Child Health and Human Development, Bethesda, MD
Description
In order to evaluate the reliability of high affinity [3H]GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in [3H]GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [3H]GABA uptake
Additional details
Publishing Information
- Journal Title
- J. Neurosci.
- Journal Volume
- 3
- Journal Issue
- 2
- Series
- J. Neurosci.
- Journal Page Range
- 376-382
- ISSN
- 0270-6474
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 16077598
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AMINOBUTYRIC ACID; AUTORADIOGRAPHY; BIOLOGICAL LOCALIZATION; CEREBRAL CORTEX; CYTOCHEMISTRY; DECARBOXYLASES; DIFFUSION; GLUTAMIC ACID; IN VITRO; MEMBRANES; MICE; NERVE CELLS; TRITIUM COMPOUNDS
- Descriptors DEC
- AMINO ACIDS; ANIMAL CELLS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENT; BODY; BRAIN; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CEREBRUM; DRUGS; ENZYMES; HYDROGEN COMPOUNDS; LYASES; MAMMALS; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RODENTS; SOMATIC CELLS; VERTEBRATES