Published November 1, 1977
| Version v1
Journal article
Determination of base ratios on sub-microgram quantities of RNA
Description
The determination of base compositions of ribonucleic acids is an important step in sequence analysis. A simple, sensitive procedure for chemically labelling RNA and its derivates which uses p-hydrazinobenzene-[35S]sulphonic acid as the radioactive labelling reagent was described previously, and makes use of [35S]sulphanilic acid. Now a routine labelling method for determining base compositions of RNA and ribooligonucleotides is presented, using the same procedure. The procedure is simple and very sensitive requiring only 0.5 μg of the polymer for at least a triplicate determination
Additional details
Identifiers
Publishing Information
- Journal Title
- FEBS Letters
- Journal Volume
- 83
- Journal Issue
- 1
- Series
- FEBS (Fed. Eur. Biochem. Soc.) Lett.
- Journal Page Range
- 81-84
- ISSN
- 0014-5793
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 9362779
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Is Lead record
- Yes
- Descriptors DEI
- CHEMICAL COMPOSITION; CHEMICAL REACTIONS; ENZYMES; LABELLED COMPOUNDS; LABELLING; NUCLEOTIDES; RNA; SULFANILIC ACID; SULFUR 35
- Descriptors DEC
- AMINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIOACTIVE MATERIALS; RADIOISOTOPES; SULFONIC ACIDS; SULFUR ISOTOPES
Optional Information
- Notes
- Updated automatically by Metadata and Full-Text Enrichment Agent