Published April 1990 | Version v1
Journal article Metadata-only

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: A potential homologue of the yeast PRP8 protein

Creators

  • 1. Massachusetts Institute of Technology, Cambridge (USA)

Description

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome

Additional details

Publishing Information

Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
87
Journal Issue
8
Series
Proc. Natl. Acad. Sci. U.S.A.
Journal Page Range
2082-2086
ISSN
0027-8424
CODEN
PNASA

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
21067200
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOCHEMICAL REACTION KINETICS; CELL NUCLEI; CROSS-LINKING; ELECTROPHORESIS; HELA CELLS; IMMUNOASSAY; MAN; MESSENGER-RNA; MOLECULAR WEIGHT; NUCLEOPROTEINS; PRECURSOR; SACCHAROMYCES CEREVISIAE; ULTRAVIOLET RADIATION
Descriptors DEC
ANIMAL CELLS; ANIMALS; CELL CONSTITUENTS; CHEMICAL REACTIONS; ELECTROMAGNETIC RADIATION; FUNGI; KINETICS; MAMMALS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; POLYMERIZATION; PRIMATES; PROTEINS; RADIATIONS; REACTION KINETICS; RNA; SACCHAROMYCES; TUMOR CELLS; VERTEBRATES; YEASTS