Effects of the radiolysis products of sennoside A on HepG2 and PC-3 cell
Creators
- 1. Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)
Description
Radiolysis of sennoside A was carried out by gamma irradiation and the anti-cancer activities of the radiolysis product were evaluated. An aqueous solution of sennoside A was exposed to 0.5-3 kGy of gamma irradiation and the radiolysis products were analyzed by HPLC. A fraction of radiolysis product (RLF) of sennoside A was isolated and the RLF was presumed as a rhein-8-β-D-glucoside. The anticancer effect of the RLF was compared with the sennoside and rhein using a in vitro assay system of human prostate cancer cells (PC-3) and human hepatoma HepG2 cells. The cell viability of PC-3 and HepG2 cell was significantly decreased to 12.4±1.2% and 32.4±2.1%, respectively, by the treatment of 0.6 μM of RLF. The sennoside A (range from 0 to 25 μM) had no cytotoxic effect on PC-3 and HepG2 cells, while the rhein had the effect on HepG2 cells with a LD50 at 80 μM
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Industry
- Journal Volume
- 10
- Journal Issue
- 2
- Series
- 26 refs, 6 figs
- Journal Page Range
- p. 83-89
- ISSN
- 1976-2402
INIS
- Country of Publication
- Korea, Republic of
- Country of Input or Organization
- Korea, Republic of
- INIS RN
- 48049778
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- AQUEOUS SOLUTIONS; GAMMA RADIATION; NEOPLASMS; PROSTATE; RADIOLYSIS; TUMOR CELLS
- Descriptors DEC
- ANIMAL CELLS; BODY; CHEMICAL RADIATION EFFECTS; CHEMICAL REACTIONS; DECOMPOSITION; DISEASES; DISPERSIONS; ELECTROMAGNETIC RADIATION; GLANDS; HOMOGENEOUS MIXTURES; IONIZING RADIATIONS; MALE GENITALS; MIXTURES; ORGANS; RADIATION EFFECTS; RADIATIONS; SOLUTIONS