A study on adrenomedullin radioimmunoassay kit and its clinical applications

Objective: To find a radioimmunoassay (RIA) method of adrenomedullin (ADM) and its clinical applications. Methods: The serum ADM antibody was prepared. ASM was labelled by the chloramine-T method. Concentration of serum ADM of coronary heart disease, hypertension patients and normal subjects were measured with this RIA method. The uptakes of 125I-ADM of different tissues of mice were measured six hours after intravenous 125I-ADM administration. Results: The results showed that the concentrations of serum ADM in coronary heart disease, hypertension patients was significantly higher than that in normal subjects. From higher to lower, the order of 125I-ADM uptakes in different tissues was as follows: kidney, lung, angio-endothelial, pancreatic, brain, and smooth muscle tissue. The concentration of ADM in the supernatants of mouse angio-endotheliocyte and smooth muscle cell cultures at different intervals were 46.54 ng/L and 6.71 ng/L and the highest value is at 24 h. Conclusions: RIA-ADM can offer a reliable method for study, diagnosis and treatment of coronary heart disease, hypertension in clinical practising