Published September 29, 1986
| Version v1
Journal article
Enzymatic quantification of strand breaks of DNA induced by vacuum-UV radiation
Creators
- 1. Tokyo Univ. (Japan). Dept. of Physics
- 2. Research Lab., Kochi Medical School, Nankoku (Japan)
Description
Hind3 digested plasmid DNA dried on an aluminum plate was irradiated by vacuum-UV at 160 and 195 nm using a synchrotron irradiation system. A change induced in the DNA, presumably a single strand break, was quantified by the aid of the strand break-derived stimulation of poly(ADP-ribose) synthetase activity. The end group of strand breaks so induced was recognized by the enzyme as effectively as that by DNase 1 treatment, suggesting a nicking as the major lesion inflicted on the DNA. The fluence (UV) dependent stimulation of poly(ADP-ribose) synthetase activity was much higher upon 160 nm irradiation than upon 195 nm irradiation. (Auth.)
Additional details
Publishing Information
- Journal Title
- FEBS (Fed. Eur. Biochem. Soc.) Lett.
- Journal Volume
- 206
- Journal Issue
- 1
- Series
- FEBS (Fed. Eur. Biochem. Soc.) Lett.
- Journal Page Range
- 151-153
- ISSN
- 0014-5793
- CODEN
- FEBLA
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 18031341
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- DNA; FAR ULTRAVIOLET RADIATION; GENETIC RADIATION EFFECTS; LIGASES; PLASMIDS; STRAND BREAKS; SYNCHROTRON RADIATION
- Descriptors DEC
- BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BREMSSTRAHLUNG; CELL CONSTITUENTS; ELECTROMAGNETIC RADIATION; ENZYMES; GENETIC EFFECTS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; ULTRAVIOLET RADIATION
Optional Information
- Notes
- 11 refs.; 1 figure.