Binding of 3H-diazepam with brain synaptic membranes during the development of generalized epileptic activity

The authors study the effect of the development of generalized epileptic activity in the rat cerebral cortex on binding of tritium-diazepam with synaptic membranes. The experiments showed that dependence of the quantity of tritium-diazepam bound with synaptic membranes on the concentration of label in the incubation medium is linear in character on a Scatchard plot. This is evidence of the existence of only one type of binding site withK /SUB d/ = 4.40 plus or minus 0.15 nM and B /SUB max/ = 410 plus or minus 35 fm fmoles/mg protein