Studies on in vivo radioprotection efficacy of indazolone derivative on electron beam induced oxidative stress using Drosophila Melanogaster

Indazole nucleus is an effective pharmacophore in medicinal chemistry and shows diverse biological and pharmacological activities. Indazolone and its derivatives show a wide spectrum of pharmacological activities as some of them act as dopamine antagonists, anti-inflammatory, analgesic and antipyretic agents. Benzyl-1H-indazole-3-carboxilic acids has antispermatogenetic and anticancer activity. In the present study the synthesis of 4-(4-methoxyphenyl)-6-phenyl-1,2,4,5-tetrahydro-3H-indazol-3-one was carried out and characterized by spectral and analytical data. The synthesized indazolone derivative showed good in vitro antioxidant activity namely, DPPH radical scavenging assay, FRAP assay etc. By keeping the antioxidant property in mind the synthesized molecule was screened for its in vivo radioprotection activity using Drosophila Melanogaster (Oregon K). The compound was supplied to flies in the form of diet and the safety evaluation was carried out for 7 days at 300 μg/ml concentration. Later the electron beam irradiated flies were assayed for oxidative stress markers namely, Thiobarbituric acid reactive substances (TBARS), enzymatic and Non enzymatic antioxidants.The results showed that the indazolone molecule exhibited good in vivo radioprotection activity. (author)