Published February 9, 1987 | Version v1
Journal article

NMR study of the interaction of P-450 with 4-methoxypyridine

  • 1. Academy of Sciences of the USSR, Novosibirsk. Inst. of Clinical and Experimental Medicine
  • 2. AN SSSR, Novosibirsk. Inst. Khimicheskoj Kinetiki i Goreniya

Description

Longitudinal relaxation (T1) measurements for all lines (α-CH, β-CH, O-CH3) in the 4-methoxypyridine 1H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed T1-1 value was determined from its dependence on 4-methoxypyridine concentration. In the P-450-4-methoxypyridine complex the latter is oriented so that the nitrogen of pyridine is directed towards the Fe3+. (Auth.)

Additional details

Publishing Information

Journal Title
FEBS (Fed. Eur. Biochem. Soc.) Lett.
Journal Volume
212
Journal Issue
1
Series
FEBS (Fed. Eur. Biochem. Soc.) Lett.
Journal Page Range
53-57
ISSN
0014-5793
CODEN
FEBLA

Optional Information

Notes
18 refs.; 3 figs.; 2 tabs.