Effects of Ku80 on radiosensitivity and cell cycle in human esophageal carcinoma cells
Creators
- 1. Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin (China)
Description
Objective: To study the role of Ku80 in the treatment of esophageal cancer through inhibiting the Ku80 expression by shRNA. Methods: shRNA-Ku80 vector was constructed. The effectiveness and feasibility of RNA interference were confirmed by Western blot and RT-PCR methods. The cell sensitivity to gamma-rays was studied by colony formation assay. The effects of shRNA-Ku80 on cell cycle were observed by flow cytometry analysis. Results: ShRNA-Ku80 vector was constructed successfully. Inhibition of Ku80 expression by shRNA enhanced the sensitivity of esophageal cancer cells to gamma-rays, shRNA K3 or shRNA H2 showed higher percentage of cells in G2/M phase (61.8% vs 28.6%; 64.3% vs 28.6%). Conclusions: Inhibitions of Ku80 expression by shRNA play a role in the treatment of esophageal cancer. Ku80 might be a new target of tumor treatment. (authors)
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 29
- Journal Issue
- 1
- Journal Page Range
- p. 44-46
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 41095478
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- CARCINOMAS; CELL CYCLE; COLONY FORMATION; ESOPHAGUS; GAMMA RADIATION; HUMAN POPULATIONS; INHIBITION; INTERFERENCE; POLYMERASE CHAIN REACTION; PROTEINS; RADIOSENSITIVITY; RNA; TUMOR CELLS
- Descriptors DEC
- ANIMAL CELLS; BODY; DIGESTIVE SYSTEM; DISEASES; ELECTROMAGNETIC RADIATION; GENE AMPLIFICATION; IONIZING RADIATIONS; NEOPLASMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; POPULATIONS; RADIATIONS; SENSITIVITY
Optional Information
- Notes
- 3 figs., 1 tab., 13 refs.