Published November 1992 | Version v1
Journal article Metadata-only

Testing of the enzyme nuclease S1 for recognition of the defects of DNA secondary structure

Creators

  • 1. AN SSSR, Moscow (Russian Federation). Inst. Khimicheskoj Fiziki

Description

In order to develop an adequate method for quantitative determination of age-related defects of DNA secondary structure in somatic cells, the testing of enzyme nuclease S1 (Sigma) was carried out. The conditions have been selected under which this enzyme revealed all single-stranded breaks and did not induce nonspecific breaks in DNA. It was shown that homopurine-homopyridine blocks of DNA capable of changing to H-form hypersensitive to S1, were not detected with the enzyme in the selected conditions

Additional details

Additional titles

Original title (Russian)
Испытания фермента нуклеазы S1 для опознания дефектов вторичной структуры ДНК

Publishing Information

Journal Title
Izvestiya Akademii Nauk - Rossijskaya Akademiya Nauk. Seriya Biologicheskaya
Journal Issue
no.6
Journal Page Range
p. 937-940.
ISSN
0002-3329

INIS

Country of Publication
Russian Federation
Country of Input or Organization
Russian Federation
INIS RN
25002615
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; DNA; METABOLISM; NUCLEASES; RADIATION DOSES; SOMATIC CELLS; STRAND BREAKS; X RADIATION
Descriptors DEC
ANIMAL CELLS; BIOLOGICAL EFFECTS; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; HYDROLASES; IONIZING RADIATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PROTEINS; RADIATION EFFECTS; RADIATIONS