γ-H2AX as protein bio marker for radiation exposure

For large scale exposures of the human population to ionising radiation, there is a need for cost-effective high throughput assessment of radiation exposure levels from biological samples to allow triage decisions to be made. Here we discuss the usefulness of the DNA damage marker γ-H2AX for this purpose. Foci of γ-H2AX form in response to radiation-induced DNA double strand breaks and can be quantified by immunofluorescence microscopy or flow cytometry. Several studies have analysed this marker in patients' blood samples to determine radiation exposures during various diagnostic or therapeutic radiation treatments. Such planned exposures involve only a moderate number of samples which can be obtained at a prearranged time following exposure. In contrast, application of this method as a triage tool in large scale radiological emergencies demands high throughput sample processing and analysis. The rapid kinetics of γ-H2AX induction and loss presents a major challenge to its successful application as a triage tool. These and other as yet unresolved questions are discussed.