Development of rast assay for determination of anti-popolus canadensis IgE antibodies
Creators
- 1. Belgrade Univ. (Yugoslavia)
- 2. Belgrade Univ. (Yugoslavia). Medicinski Fakultet
- 3. Belgrade Univ. (Yugoslavia). Mikrobiologicheskij Inst.
- 4. Belgrade Univ. (Yugoslavia). Inst. of Electrochemistry
Description
One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb E1 was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mL extract is needed for coupling of Ig BrCN activated paper discs. Coupling was performed in Na2CO3/NaHCO3 buffer pH 10, on 4 deg C for 48h. Using this newly formed RAST, specific for Populus canadensis, we have determinated anti-Populus canadensis IgE antibodies as well as cross reactivity between pollens of Populus canadensis and Populus deltoides. (author). 12 refs., 3 figs., 1 tab
Additional details
Publishing Information
- Journal Title
- Journal of Radioanalytical and Nuclear Chemistry
- Journal Volume
- 206
- Journal Issue
- 1
- Journal Page Range
- p. 145-149.
- ISSN
- 0236-5731
- CODEN
- JRNCDM
Conference
- Title
- International Conference on Isotopes.
- Dates
- 7-12 May 1995.
- Place
- Beijing (China).
INIS
- Country of Publication
- Hungary
- Country of Input or Organization
- Hungary
- INIS RN
- 27074820
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- ALLERGY; BROMINE COMPOUNDS; CYANIDES; ENZYME INHIBITORS; IODINE 125; LABELLING; MONOCLONAL ANTIBODIES; POLLEN; SAMPLE PREPARATION; TITRATION
- Descriptors DEC
- ANTIBODIES; BETA DECAY RADIOISOTOPES; CHEMICAL ANALYSIS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GAMETES; GERM CELLS; HALOGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; PATHOLOGICAL CHANGES; QUANTITATIVE CHEMICAL ANALYSIS; RADIOISOTOPES; VOLUMETRIC ANALYSIS