Study on selection of a new strawberry strain of Benihoppe developed by ⁶⁰Co-γ irradiation induced mutation breeding

In order to expand the methods of strawberry breeding, used strawberry cultivar Benihoppe as the experimental materials and explored the methods of radiation mutation combined with tissue culture breeding technology. The leaves callus of Benihoppe were treated with different irradiation dose of ⁶⁰Co-γ rays, and the high-quality mutant strawberry line 2010-30 was obtained by directional screening. The agronomic traits, nutritional quality, stigma receptivity and anther viability of Benihoppe and 2010-30 were compared. The results showed that the optimum irradiation dose was 20 Gy. 2010-30 exhibited higher contents of vitamin C (0.663 mg · g^-1) and soluble solid (11.65%) and higher fruit hardness (571.71 g). The stigma receptivity of 2010-30 was higher than Benihoppe. The pollen abortion rate of 2010-30 was lower than that of Benihoppe. The mutant strain 2010-30 obtained by ⁶⁰Co-γ irradiation can be used as a new germplasm resources for strawberry breeding, and it can be applied in production. The result provides a technical reference and theoretical basis for increasing genetic variation, creating new germplasm materials, breeding new varieties and exploring breeding technology by combining radiation mutagenesis in connection with tissue culture on strawberry. (authors)