Performance evaluation of ²²⁴Ra/²¹²Pb generators used for ²¹²Pb -peptide preparations as a TAT agent for clinical use

One attractive radionuclide for this purpose is Pb-212, which by itself is a β-emitter, but acts as an in vivo generator for its short-lived α-emitting daughters. We hereby, report the usage of ²²⁴Ra/²¹²Pb generator system with an aim to evaluate the feasibility as well as the performance of ²²⁴Ra/²¹²Pb generators to be used for radiosynthesis of ²¹²Pb peptides for Targeted alpha therapy. The generator had a shelf life of 1 week and was be eluted 2-3 times at an interval of 24 hrs. The mean average time elapsed from the day of manufacture of the generator to the day of first elution was 120hrs. The average yield of Pb-212 along with daughters from the generator in 1st elution was noted to be 67.6% which mounted to 73.5% in the 2nd elution. The purified Pb-212 was used for the labeling with peptide and the final yield of the radiolabelled product obtained was 91%. The RCP of radiopharmaceuticals as determined by thin layer chromatography was 98 ± 0.22%. The generator represents a feasible, straight forward and promising method for efficient production of Pb-212 without the use of sophisticated machinery, time-consuming processes, or hazardous chemicals which can be efficiently utilized for further radiolabeling with peptides as targeted alpha therapy agent for clinical use