Genetic studies of the HLA-D region by the primed lymphocyte test
Description
The control and influence of the stimulating DRw antigens on primed lymphocyte typing (PLT) response was studied by using a discriminatory PLT assay with a low responder: stimulator ratio. Positive restimulation was established at 90.3% RR, based on a statistical evaluation of a composite or %RR values from 13 separate intrafamily PLTs performed in this laboratory. Two intrafamily PLT cells were then made against specificities HLA-DRwl and HLA-DRw3 and restimulated with a panel of unrelated individuals. The results show a very high correlation (p < 0.001) between the HLA-DRw antigen specificity of those unrelated panel cells that stimulated in PLT and the HLA-DRw target specificity, which shows that PLT reactivity is strongly influenced by HLA-DRw
Additional details
Additional titles
- Augmented title (English)
- Gamma radiation
Publishing Information
- Journal Title
- Transplant. Proc.
- Journal Volume
- 10
- Journal Issue
- 4
- Series
- Transplant. Proc.
- Journal Page Range
- 775-779
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 10460961
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ANTIGENS; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; GAMMA RADIATION; GENETICS; IMMUNE REACTIONS; LYMPHOCYTES
- Descriptors DEC
- ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGY; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LEUKOCYTES; RADIATION EFFECTS; RADIATIONS; SOMATIC CELLS