Published July 23, 2014
| Version v1
Journal article
Purification, crystallization and preliminary X-ray crystallographic studies of Rv3705c from Mycobacterium tuberculosis
- 1. East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of (China)
- 2. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of (China)
- 3. Center for Tuberculosis Control of Guangdong Province, Guangzhou, People's Republic of (China)
Description
The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of Rv3705c from M. tuberculosis are described. The conserved protein Rv3705c from Mycobacterium tuberculosis has been cloned, expressed, purified and crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as a precipitant. The Rv3705c crystals exhibited space group P6122 or P6522, with unit-cell parameters a = b = 198.0, c = 364.1 Å, α = β = 90, γ = 120°, and diffracted to a resolution of 3.3 Å
Availability note (English)
Available from http://dx.doi.org/10.1107/S2053230X14014113; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118811Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118811;
- DOI
- 10.1107/S2053230X14014113;
- PII
- S2053230X14014113;
Publishing Information
- Journal Title
- Acta crystallographica. Section F, Structural biology communications
- Journal Volume
- 70
- Journal Issue
- Pt 8
- Journal Page Range
- p. 1090-1092
- ISSN
- 2053-230X
- CODEN
- ACSFEN
INIS
- Country of Publication
- United States
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46081140
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Descriptors DEI
- CRYSTALLIZATION; CRYSTALS; DIFFUSION; PROTEINS; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION
- Descriptors DEC
- COHERENT SCATTERING; DIFFRACTION; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; SCATTERING; SYMMETRY GROUPS
Optional Information
- Copyright
- Copyright (c) Lu et al. 2014
- Notes
- PMCID: PMC4118811; PMID: 25084389; PUBLISHER-ID: hc5173; OAI: oai:pubmedcentral.nih.gov:4118811; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.