Published March 2007
| Version v1
Journal article
Two-round enzyme-amplified lanthanide luminescence time-resolved fluorescence assay for nucleic acid hybridization
Creators
- 1. Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Beijing Union Medical College, Tianjin (China)
Description
Objective: To investigate a supersensitive for nucleic acid hybridization assay. Methods: Using the two-roung enzyme-amplified action, PCR amplification, Tb chelate and time-resolved detection technology, target PSA DNA was detected. Results: The range of the standard curve for detecting target PSA DNA is more than two order of magnitude; the sensitivity was 10pmol/L (0.5fmol/well); the accuracy and the precision was 77%-95% and 12.9%-15.3% respectively when the target PSA DNA was 10, 30 and 60 pmol/L. Conclusion: The method is a supersensitive and new assay technology which has a good prospect of applications. (authors)
Additional details
Publishing Information
- Journal Title
- International Journal of Radiation Medicine and Nuclear Medicine
- Journal Volume
- 31
- Journal Issue
- 2
- Journal Page Range
- p. 70-73
- ISSN
- 1673-4114
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 40041054
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ACCURACY; AMPLIFICATION; CHELATES; DETECTION; DIAGRAMS; DNA; ENZYMES; FLUORESCENCE; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; RARE EARTHS; SENSITIVITY; STANDARDS; TIME RESOLUTION; USES
- Descriptors DEC
- BIOTECHNOLOGY; COMPLEXES; ELEMENTS; EMISSION; GENE AMPLIFICATION; GENETIC ENGINEERING; INFORMATION; LUMINESCENCE; METALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOTON EMISSION; PROTEINS; RESOLUTION; TIMING PROPERTIES
Optional Information
- Notes
- 3 figs., 7 refs.