Published May 1998 | Version v1
Journal article

Efficient multiple-solvent suppression for the study of the interactions of organic solvents with biomolecules

Description

A modification of the excitation sculpting sequence [Hwang, T.-L. and Shaka, A.J. (1995) J. Magn. Reson., A112, 275-279] for achieving efficient multiple-solvents suppression in 1D and 2D 1H NMR experiments is presented. Implementation of this scheme in the ePHOGSY experiment allows rapid and sensitive detection of weak interactions between organic solvents or small molecules and biomolecules. Application of the techniques to the peptide Sandostatin (registered) dissolved in H2O and DMSO is demonstrated

Additional details

Identifiers

Publishing Information

Journal Title
Journal of Biomolecular NMR
Journal Volume
11
Journal Issue
4
Journal Page Range
p. 437-444
ISSN
0925-2738

INIS

Country of Publication
Netherlands
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
39105472
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
DMSO; EXCITATION; INHIBITION; NUCLEAR MAGNETIC RESONANCE; ORGANIC SOLVENTS; PEPTIDES; WEAK INTERACTIONS
Descriptors DEC
BASIC INTERACTIONS; ENERGY-LEVEL TRANSITIONS; INTERACTIONS; MAGNETIC RESONANCE; NONAQUEOUS SOLVENTS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PROTEINS; RESONANCE; SOLVENTS; SULFOXIDES

Optional Information

Copyright
Copyright (c) 1998 Kluwer Academic Publishers