Vitamin B1 resorption. X.Changes in thiamine resorption in the small intestine of chickens and rats treated with somatotropic hormone

A trial was carried out with broiler chickens aged one month and Wistar male albino rats of mean liveweight of 207 g. The chickens were intramusculary injected with somatotropic hormone (STH), Sotropin H (DDR) 2 units/1O0 g liveweight for 3 successive days and the rats - 4 days. Intestine alkaline phosphatase activity was determined for a separate group of chickens post hormone injection. The resorption of 35S-thiamine, introduced in bilaterally ligated duodenum, 30 μCi/kg liveweight, was studied in chickens; in rats -the resorption of thiamine chloride (non radioactive), 20O μg dissolved in 3 ml volume, introduced in the lupen of the entire small intestine (duodenum, jejunum, ileum) bilaterally ligated. It was found that STH enhanced thiamine both in chickens and rats (p < 0.001). To the increased intestine alkaline phosphatase activity (EC 3.1.3.1) induced by STH corresponds higher thiamine resorption. These results support data earlier published on a possible participation of alkaline phosphatase in vitamin B1 resorption in the small intestine (2, 3). Thiamine resorption depends on the activity of metabolic processes in small intestine epithelium. This is confirmed by data on the effect of sodium fluoride (NaF) and monoiodoacetic acid (MIAA). Thiamine resorption is depressed when 10-2M or 1O-3M MIAA are jointly introduced in the lumen of the intestine. When the action of NaF and MIAA takes place against the background of STH action, then thiamine resorption goes up and equal that of the control group. NaF inhibits small intestine respiratory activity. These results are interpreted in the sense that the active thiamine transport is influenced by STH which restores the enzyme and respiratory activity of intestine epithelial cells. (author)