Peak-fitting analysis with gaussian line shapes against experimental RSE spectrum consisting of multiple peak

Relaxation time of an unpaired electron on free radicals induced in irradiated foods is one of the indicators for radical interactions. Using continuous wave (CW-) ESR, Lund's simulation is also utilized for the relaxation time of a single peak. Peak-fitting analysis was carried out prior to calculate the relaxation time of multiple peak of ESR spectra. The relaxation time can be directly measured on pulsed ESR. When the Lund's simulation was applied for multiple peak of irradiated amino acid, the relaxation time was quite different from the time measured on pulsed ESR. The ESR spectrum of the amino acid was resolved to several Gaussian peaks, and the sets of parameters obtained from each Gaussian peak were applied to the Lund's simulation. The calculated T₂ values were in good agreement with pulsed ESR values. When ESR spectrum consisting of multiple peak is recorded on CW-ESR, the peak-fitting analysis following Lund's simulation is effective to calculate the relaxation time of each peak of the multiple peak. When two peaks were located in same g-factor on pulsed ESR, relaxation time from CW-ESR measurements can be easy estimated after the peak-fittings based on radical structure. (author)