Published 2015 | Version v1
Journal article Metadata-only

Molecular characterization of two accessions of Cydonia oblonga

Creators

  • 1. Instituto Tecnologico de Costa Rica (ITCR), Escuela de Biologia(Costa Rica)

Description

The use of the trnH-psbA plastid spacer and the matK coding sequence were evaluated as possible markers for the molecular characterization of two accessions quince (C. oblonga) from Argentina and Costa Rica. The research was carried out by the Centro de Investigacion en Biotecnologia del Instituto Tecnologico de Costa Rica (ITCR), during the year 2014. The amplified products were analyzed with MEGA v6.0 software to calculate the genetic distances using the Tamura-Nei model . The matK sequence presented the highest percentage of variable sites and intraspecific distance, indicating the presence of polymorphisms between the accessions. The use of the trnH-psbA spacer for quince had little capacity for discrimination because at the intra-specific level this locus presented less divergence. (author)

Availability note (English)

Also available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica; Also available on line: https://revistas.ucr.ac.cr/index.php/agromeso/article/view/19329

Abstract (Spanish)

El uso del espaciador plastidico trnH-psbA y la secuencia codificante matK fueron evaluados como posible marcadores para la caracterizacion molecular de dos accesiones de membrillo (C. oblonga), provenientes de Argentina y de Costa Rica. La investigacion fue realizada por el Centro de Investigacion en Biotecnologia del Instituto Tecnologico de Costa Rica (ITCR), durante el ano 2014. Los productos amplificados fueron analizados con el software MEGA v6.0 para calcular las distancias geneticas mediante el modelo de Tamura-Nei. La secuencia matK ha presentado el mayor porcentaje de sitios variables y distancia intraespecifica, indicando la presencia de polimorfirmos entre las accesiones. La utilizacion del espaciador trnH-psbA para membrillo, tuvo poca capacidad de discriminacion debido a que a nivel intraespecifico este locus presento menos divergencias. (autor)

Additional details

Additional titles

Original title (Spanish)
Caracterizacion molecular de dos accesiones de Cydonia oblonga

Identifiers

URL
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/19329;

Publishing Information

Journal Title
Agronomia Mesoamericana
Journal Volume
26
Journal Issue
no.2
Journal Page Range
p. 351-354
ISSN
1021-7444

INIS

Country of Publication
Costa Rica
Country of Input or Organization
Costa Rica
INIS RN
48069945
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
BIOLOGICAL MARKERS; BIOTECHNOLOGY; DNA; EXPERIMENTAL DATA; FRUIT TREES; GENETICS; GENOTYPE; MOLECULAR MODELS; MOLECULAR STRUCTURE; OPTIMIZATION; PEARS; ROSACEAE; SPECTROPHOTOMETRY
Descriptors DEC
BIOLOGY; DATA; FOOD; FRUITS; INFORMATION; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MATHEMATICAL MODELS; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; PLANTS; TREES

Optional Information

Notes
Figs., tabs., refs.