Published 2014 | Version v1
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Comparison of susceptibility to antibiotics of Staphylococcus aureus isolated from fresh cheeses and clinical samples from the Area Metropolitana and evaluation of the presence of Listeria monocytogenes in the same foodstuff

Creators

Description

60 samples of fresh cheeses were analyzed from the central markets of Alajuela, Heredia, Cartago and San Jose. Staphylococcus aureus, total coliform and thermotolerant counts were performed, as well as Escherichia coli by the NMP technique. The presence of Listeria monocytogenes was evaluated in 25 g of cheese. The confirmatory identification and sensitivity profile of S. aureus strains were obtained using the Vitek system. L. monocytogenes were identified by means of biochemical tests. The statistical package Statistical Package for the Social Sciences (SPSS) and Excell were used to analyze the data. The presence of fecal contamination was identified as well as S. aureus in fresh cheeses from the four provinces studied. The comparative analysis of S. aureus antibiotic susceptibility profiles from clinical specimens and cheeses, for the majority of antibiotics under study was able to determine that the sensitivity patterns shown have been different. The clinical strains of origin could be observed with a pattern of multiresistance more marked than the strains of alimentary origin. The presence of L. monocytogenes was identified in an important percentage of cheese samples. (author)

Availability note (English)

Available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica

Abstract (Spanish)

60 muestras de quesos frescos fueron analizadas provenientes de los mercados centrales de Alajuela, Heredia, Cartago y San Jose. Recuentos de Staphylococcus aureus, coliformes totales y termotolerantes fueron realizados, asi como Escherichia coli por la tecnica de NMP. La presencia de Listeria monocytogenes fue evaluada en 25 g de queso. La identificacion confirmatoria y el perfil de sensibilidad a antibioticos de las cepas de S. aureus fueron obtenidas utilizando el sistema Vitek. L. monocytogenes fueron identificadas por medio de pruebas bioquimicas. Los datos fueron analizados utilizando el paquete estadistico Statistical Package for the Social Sciences (SPSS) y Excell. La presencia de contaminacion fecal fue identificada asi como S. aureus en los quesos frescos procedentes de las cuatro provincias estudiadas. El analisis comparativo de los perfiles de sensibilidad a los antibioticos de S. aureus provenientes de muestras clinicas y quesos, para la mayoria de antibioticos en estudio fue logrado determinar que los patrones de sensibilidad mostrados han sido distintos. Las cepas de origen clinico pueden ser observadas con un patron de multirresistencia mas marcado que las cepas de origen alimentario. La presencia de L. monocytogenes fue identificada en un porcentaje importante de muestras de queso. (autor)

Additional details

Additional titles

Original title (Spanish)
Comparacion de la susceptibilidad a antibioticos de Staphylococcus aureus aislados a partir de quesos frescos y muestras clinicas del Area Metropolitana y evaluacion de la presencia de Listeria monocytogenes en el mismo producto alimenticio

Publishing Information

Imprint Place
San Jose (Costa Rica)
Imprint Pagination
50 p.

INIS

Country of Publication
Costa Rica
Country of Input or Organization
Costa Rica
INIS RN
48047488
Subject category
S60: APPLIED LIFE SCIENCES; S54: ENVIRONMENTAL SCIENCES;
Resource subtype / Literary indicator
Numerical Data, Thesis, Non-conventional Literature
Descriptors DEI
ANTIBIOTICS; BIOCHEMISTRY; CHEESE; COLIFORMS; CONTAMINATION; FECES; FOOD INDUSTRY; FOOD PROCESSING; MICROBIAL DRUG RESISTANCE; PATHOGENS; PUBLIC HEALTH; QUALITY CONTROL; STAPHYLOCOCCUS; STATISTICAL DATA; STRAINS; TESTING
Descriptors DEC
ANTI-INFECTIVE AGENTS; BACTERIA; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; CHEMISTRY; CONTROL; DATA; DRUGS; FOOD; INDUSTRY; INFORMATION; MATERIALS; MICROORGANISMS; MILK PRODUCTS; NUMERICAL DATA; ORGANIC COMPOUNDS; PROCESSING; WASTES

Optional Information

Notes
Tabs., refs.