Surface molecularly imprinted thermo-sensitive polymers based on light-weight hollow magnetic microspheres for specific recognition of BSA

Effective specific recognition of bovine serum albumin (BSA) was accomplished by the thermo-sensitive imprinted microspheres composed of N-isopropylacrylamide (NIPAM), methacrylic acid (MAA) and N,N-methylenebisacrylamide (MBA). These imprinted microspheres were prepared in the presence of hollow Fe₃O₄ microspheres as substrates via surface molecular imprinting technique (MIT) and grafting copolymerization method. In the imprinting system, hollow Fe₃O₄ microspheres possessed the distinctive properties of excellent water dispersibility, light weight and high specific surface area. These potential benefits not only reduced the agglomeration of imprinted microspheres, but also increased the effective imprinting area for BSA. And owing to the existence of poly-(N-isopropylacrylamide) (pNIPAM), the fabricated molecularly imprinted polymers (MIPs) were highly sensitive to ambient temperature and played a significant role in efficient recognition for BSA. When treated at 32 °C, the optimal adsorption capacity (Q) and imprinting factor (IF) for BSA were obtained, 430.67 mg/g and 2.01, respectively. Combining hollow Fe₃O₄ microspheres with thermo-sensitive polymers, the surface-imprinted materials for specific capture of target protein would show great promise for bioseparation and biosensors.