HPLC assay for 2-(3-aminopropylamino)ethanethiol (WR-1065) in plasma
- 1. Southwest Research Inst., San Antonio, TX
Description
A high pressure liquid chromatography (HPLC) plasma assay for WR-1065 is described which is both precise and accurate throughout the concentration range from 1 to 500 μg/mL of plasma. The analyte is separated by HPLC and detected with a thiol specific electrochemical transducer cell. The detector response is linear over the ranges 1 to 10 μg/mL, 10 to 100 μg/mL, and 100 to 500 μg/mL. The absolute retention times for WR-1065 and WT-1729 are 9 and 12 minutes, respectively. The assay uses 100 μL of plasma and requires a total chromatography cycle time of 40 minutes. The method has been found suitable for the determination of WR-1065 in plasma from a beagle dog after i.v. administration of S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721)
Additional details
Publishing Information
- Journal Title
- Int. J. Radiat. Oncol., Biol. Phys.
- Journal Volume
- 10
- Journal Issue
- 9
- Series
- Int. J. Radiat. Oncol., Biol. Phys.
- Journal Page Range
- 1517-1520
- ISSN
- 0360-3016
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 16060477
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BEAGLES; BIOASSAY; BLOOD PLASMA; DOSE-RESPONSE RELATIONSHIPS; LIQUID COLUMN CHROMATOGRAPHY; RADIOPROTECTIVE SUBSTANCES; THIOLS
- Descriptors DEC
- ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CHROMATOGRAPHY; DOGS; DRUGS; MAMMALS; MATERIALS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PERFORMANCE TESTING; RESPONSE MODIFYING FACTORS; SEPARATION PROCESSES; TESTING; VERTEBRATES