Published January 1987
| Version v1
Journal article
Radiometric studies on the oxidation of (I-14C) fatty acids by drug-susceptible and drug-resistant mycobacteria
Description
A radiometric assay system has been used to study oxidation patterns of (l - 14C) fatty acids by drug-susceptible and drug-resistant organisms of the genus Mycobacterium (M. tuberculosis - H37Rv and Erdman, M. bovis, M. avium, M. intracellulare, M.Kansasii and M. chelonei). The organisms were inoculated in sterile reaction vials containing liquid 7H9 medium, 10% ADC enrichment and 1.0 uli of one of the (l-14C) fatty acids (butyric, hexanoic, octanoic, decanoic, lauric, myristic, palmitic, stearic, oleic, linoleic, linolenic). Vials were incubated at 370C and the 14CO2 envolved was measured daily for 3 days with a Bactec R-301 instrument. (M.A.C.)
Abstract (Portuguese)
Estudam-se os padroes de oxidacao dos (l-14C) acidos graxos por microorganismos do genero Mycobacterium sensiveis e resistentes a drogas, atraves de um sistema radiometrico. Utilizam-se M. tuberculosis (cepa H37Rv e Erdman), M. bovis, M. avium, M. intracellulare, M. Kansasii e M. chenolei. Inoculam-se as micobacterias em frascos estereis contendo o meio liquido 7H9 com 10% do complexo albumina-dextrose-catalase e 1,0 uli de um dos (l-14C) acidos graxos (butirico, hexanoico, ortanoico, decanoico, laurico, miristico, plamitico, estearico, oleico, linoleico, linolenico). Incubam-se os frascos a 370C e mede-se o 14CO2 produzido com uma maquina Bactec-R-301. (M.A.C.)Additional details
Publishing Information
- Journal Title
- Rev. Inst. Med. Trop. Sao Paulo
- Journal Volume
- 29
- Journal Issue
- 1
- Series
- Rev. Inst. Med. Trop. Sao Paulo.
- Journal Page Range
- 9-17
- ISSN
- 0036-4665
- CODEN
- RMTSA
INIS
- Country of Publication
- Brazil
- Country of Input or Organization
- Brazil
- INIS RN
- 19083141
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CARBON 14; CARBOXYLIC ACIDS; MYCOBACTERIUM; OXIDATION; RADIOMETRIC ANALYSIS
- Descriptors DEC
- BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON ISOTOPES; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; EVEN-EVEN NUCLEI; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; QUANTITATIVE CHEMICAL ANALYSIS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES